High through put analysis of MALDI time-of-flight MS/MS and LCMS/MS data
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Design, Construction and Calibration of a Laser Ionization Time-of-Flight Mass Spectrometer
A time-of-flight mass spectrometer (TOF-MS) developed in our laboratory at Isfahan University of Technology is described here. The TOF-MS instrument uses laser as the ionization source which provides an opportunity to investigate the ions formed in laser ablation or desorption. The TOF-MS has an ionization chamber containing an accelerator and an ion lens to focus the ions into a one meter line...
متن کاملStudies of Oligo- and Polysaccharides by MALDI-TOF and ESI-ITMS Mass Spectrometry
Broberg, S. Studies of Oligoand Polysaccharides by MALDI-TOF and ESI-IT MS Mass Spectrometry. Doctor’s dissertation. ISSN 1401-6249, ISBN 91-576-6465-X This thesis presents methods for analyzing carbohydrates with matrix-assisted laser desorption/ionization time-of-flight, MALDI-TOF, and electrospray ionization ion trap multiple-stage, ESI-IT MS, mass spectrometry, MS. MALDI-TOF MS was employed...
متن کاملIdentification accuracy of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for clinical pathogenic bacteria and fungi diagnosis: a meta-analysis
Recently, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been enthusiastically adopted in clinical microbial identification by virtue of its rapid process, easy operation, relatively low cost and high throughput. However, its identification accuracy for common clinical pathogens is still inconclusive. Therefore, we conducted this meta-analysis to...
متن کاملIdentification of mammalian cell lines using MALDI-TOF and LC-ESI-MS/MS mass spectrometry.
Direct mass spectrometric analysis of complex biological samples is becoming an increasingly useful technique in the field of proteomics. Matrix-assisted laser desorption/ionization mass spectroscopy (MALDI-MS) is a rapid and sensitive analytical tool well suited for obtaining molecular weights of peptides and proteins from complex samples. Here, a fast and simple approach to cellular protein p...
متن کاملRapid, Sensitive, and Specific Escherichia coli H Antigen Typing by Matrix-Assisted Laser Desorption Ionization-Time of Flight-Based Peptide Mass Fingerprinting.
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has gained popularity in recent years for rapid bacterial identification, mostly at the genus or species level. In this study, a rapid method to identify the Escherichia coli flagellar antigen (H antigen) at the subspecies level was developed using a MALDI-TOF MS platform with high specificity and sensit...
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تاریخ انتشار 2001